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- Title
Decrease in Akt/PKB signalling in human skeletal muscle by resistance exercise.
- Authors
Deldicque, Louise; Atherton, Philip; Patel, Rekha; Theisen, Daniel; Nielens, Henri; Rennie, Michael J.; Francaux, Marc
- Abstract
We analysed the effects of resistance exercise upon the phosphorylation state of proteins associated with adaptive processes from the Akt/PKB (protein kinase B) and the mitogen-activated protein kinase (MAPK) pathways. Nine healthy young men (21.7 +/- 0.55 year) performed 10 sets of 10 leg extensions at 80% of their 1-RM (repetition maximum). Muscle biopsies were taken from the vastus lateralis at rest, within the first 30 s after exercise and at 24 h post-exercise. Immediately post exercise, the phosphorylation states of Akt/PKB on Thr308 and Ser473 and 4E-BP1 on Thr37/46 (eukaryotic initiation factor 4E-binding protein 1) were decreased (-60 to -90%, P < 0.05). Conversely, the phosphorylation of p70(s6k) (p70 ribosomal S6 kinase) on Thr421/Ser424 was increased more than 20-fold (P < 0.05), and this was associated with a 10- to 50-fold increase in the phosphorylation of p38 and ERK1/2 (extracellular signal-regulated kinase) (P < 0.05). Twenty-four hours post-exercise the phosphorylation state of Akt/PKB on Thr308 was depressed, whereas the phosphorylation of p70(s6k) on Thr421/Ser424 and sarcoplasmic ERK1/2 were elevated. The present results indicate that high-intensity resistance exercise in the fasted state inhibits Akt/PKB and 4E-BP1 whilst concomitantly augmenting MAPK signalling and p70(s6k) on Thr421/Ser424.
- Subjects
CHEMICAL reactions; PROTEIN kinases; PHOSPHOTRANSFERASES; CHEMICAL processes; CLINICAL pathology; CARRIER proteins; CELL lines; CELLULAR signal transduction; COMPARATIVE studies; EXERCISE; FASTING; RESEARCH methodology; MEDICAL cooperation; MUSCLE contraction; PHOSPHOPROTEINS; PHOSPHORYLATION; RESEARCH; RESEARCH funding; STEM cells; TRANSFERASES; EVALUATION research; QUADRICEPS muscle; PROTEIN kinase inhibitors; PHARMACODYNAMICS
- Publication
European Journal of Applied Physiology, 2008, Vol 104, Issue 1, p57
- ISSN
1439-6319
- Publication type
journal article
- DOI
10.1007/s00421-008-0786-7