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- Title
iPS cells produce viable mice through tetraploid complementation.
- Authors
Xiao-yang Zhao; Wei Li; Lv, Zhuo; Lei Liu; Tong, Man; Tang Hai; Hao, Jie; Chang-long Guo; Qing-wen Ma; Liu Wang; Fanyi Zeng; Qi Zhou
- Abstract
Since the initial description of induced pluripotent stem (iPS) cells created by forced expression of four transcription factors in mouse fibroblasts, the technique has been used to generate embryonic stem (ES)-cell-like pluripotent cells from a variety of cell types in other species, including primates and rat. It has become a popular means to reprogram somatic genomes into an embryonic-like pluripotent state, and a preferred alternative to somatic-cell nuclear transfer and somatic-cell fusion with ES cells. However, iPS cell reprogramming remains slow and inefficient. Notably, no live animals have been produced by the most stringent tetraploid complementation assay, indicative of a failure to create fully pluripotent cells. Here we report the generation of several iPS cell lines that are capable of generating viable, fertile live-born progeny by tetraploid complementation. These iPS cells maintain a pluripotent potential that is very close to ES cells generated from in vivo or nuclear transfer embryos. We demonstrate the practicality of using iPS cells as useful tools for the characterization of cellular reprogramming and developmental potency, and confirm that iPS cells can attain true pluripotency that is similar to that of ES cells.
- Subjects
CELLS; TRANSCRIPTION factors; LABORATORY mice; MICE anatomy; EMBRYONIC stem cells; CELL proliferation; CELL lines; GENOMES; GENETICS
- Publication
Nature, 2009, Vol 461, Issue 7260, p86
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/nature08267