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- Title
Genomic analysis of isopentenyltransferase genes and functional characterization of TaIPT8 indicates positive effects of cytokinins on drought tolerance in wheat.
- Authors
Na Wang; Jun Chen; Yuan Gao; Yongbin Zhou; Ming Chen; Zhaoshi Xu; Zhengwu Fang; Youzhi Ma
- Abstract
Cytokinins (CKs) function in plant development and during stress responses, but their role in drought tolerance in wheat is unknown. In the present study, 24 isopentenyltransferase (IPT) genes, encoding ratelimiting enzymes in CK biosynthesis were identified in the wheat genome. The chromosomal locations and structures of the genes, protein properties, and phylogenetic relationships were characterized. ATP/ADP TaIPT genes showed tissue-specific expression. TaIPT2, TaIPT7, and TaIPT8 expression was rapidly induced by 0.5-1 h drought treatments, which decreased to low levels after 2 h drought treatment, as did most other TaIPT genes. TaIPT8-5a/5b/5d triple mutants showed decreased levels of tZtype CK under normal and drought conditions and reduced drought tolerance, which, however, did not manifest as phenotype alterations. By contrast, transgenic wheat plants with drought-induced TaIPT8 showed increased drought tolerance. Our study provides a foundation for further investigation of TaIPT genes and novel insights into the role of CKs in the drought response of wheat.
- Subjects
DROUGHT tolerance; CYTOKININS; WHEAT genetics; PLANT development; PLANT hormones; ABIOTIC stress
- Publication
Crop Journal (2095-5421), 2023, Vol 11, Issue 1, p46
- ISSN
2095-5421
- Publication type
Article
- DOI
10.1016/j.cj.2022.04.010