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- Title
3D bio scaffold support osteogenic differentiation of mesenchymal stem cells.
- Authors
Ramzan, Faiza; Khalid, Shumaila; Ekram, Sobia; Salim, Asmat; Frazier, Trivia; Begum, Sumreen; Mohiuddin, Omair A.; Khan, Irfan
- Abstract
The regeneration of osteochondral lesions by tissue engineering techniques is challenging due to the lack of physicochemical characteristics and dual‐lineage (osteogenesis and chondrogenesis). A scaffold with better mechanical properties and dual lineage capability is required for the regeneration of osteochondral defects. In this study, a hydrogel prepared from decellularized human umbilical cord tissue was developed and evaluated for osteochondral regeneration. Mesenchymal stem cells (MSCs) isolated from the umbilical cord were seeded with hydrogel for 28 days, and cell‐hydrogel composites were cultured in basal and osteogenic media. Alizarin red staining, quantitative polymerase chain reaction, and immunofluorescent staining were used to confirm that the hydrogel was biocompatible and capable of inducing osteogenic differentiation in umbilical cord‐derived MSCs. The findings demonstrate that human MSCs differentiated into an osteogenic lineage following 28 days of cultivation in basal and osteoinductive media. The expression was higher in the cell‐hydrogel composites cultured in osteoinductive media, as evidenced by increased levels of messenger RNA and protein expression of osteogenic markers as compared to basal media cultured cell‐hydrogel composites. Additionally, calcium deposits were also observed, which provide additional evidence of osteogenic differentiation. The findings demonstrate that the hydrogel is biocompatible with MSCs and possesses osteoinductive capability in vitro. It may be potentially useful for osteochondral regeneration.
- Subjects
MESENCHYMAL stem cell differentiation; BONE regeneration; GENE expression; UMBILICAL cord; MESENCHYMAL stem cells; TISSUE scaffolds; POLYMERASE chain reaction
- Publication
Cell Biology International, 2024, Vol 48, Issue 5, p594
- ISSN
1065-6995
- Publication type
Article
- DOI
10.1002/cbin.12131