We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
纳米酶免疫层析试纸条法检测玉米褪绿斑驳病毒.
- Authors
符娜; 向均; 张永江; 张祥林
- Abstract
【Objective】 To develop a nanozyme immuno-chromatographic strip assay (NISA) to detect Maize chlorotic mottle virus. 【Method】 The double-antibody sandwich method was used to bind the nanozyme-labeled mouse antibody IgG to MCMV, and then to the mouse antibody on the nitrocellulose membrane quality control line.【Results】 The developed NISA and reverse transcription PCR (RT-PCR) were used to detect the grinding dilutions of corn chlorotic mottle virus gradient dilution, the sensitivity was 10-7 and 10-5 respectively; the other four viruses were used as controls for specificity. There was no cross reaction in the experiment. The test strip was used to test the test samples, and the test results were verified by RT-PCR and gene sequencing methods. The results showed that the three test results were consistent.【Conclusion】 The test strip has high sensitivity and specificity for detecting MCMV, and can be used to detect MCMV in scientific research, agricultural production units and port institutions.
- Publication
Xinjiang Agricultural Sciences, 2021, Vol 58, Issue 6, p1154
- ISSN
1001-4330
- Publication type
Article
- DOI
10.6048/j.issn.1001-4330.2021.06.021