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- Title
4种抗生素用于干木耳组织分离方法的评价.
- Authors
陈艳琦; 吕艳聪; 贾培松; 张芳芳; 田龙; 宋冰; 李玉
- Abstract
[Objective] To screen out suitable working concentration of 4 antibiotics and drying condition for dry Auricularia fruiting bodies. [Methods] In this study, fresh and dry fruiting bodies of wild and cultivated were used as materials, the dry ear was soaked in 75% alcohol mixture solution of antibiotics for tissue separation. The germination ability of different parts of Auricularia auricula was studied by microscopic observation through comparing the contamination rate and germination rate of 4 antibiotics and the dry method of Auricularia auricular. [Results] The results showed that the suitable concentration and disinfection time of the four antibiotics were: 6 mg/mL and 3-5 min for penicillin; 15 mg/mL and 1-3 min for streptomycin; 15 mg/mL and 3-5 min for ceftriaxone sodium; 1.5 mg/mL and 1-3 min for gentamicin. The fruiting bodies after drying at 65℃ for 8 hours could be isolated successfully. Mycelium germination was observed in the fertile layer, hypophyll and sterile layer after drying for 8 h at 65℃. [Conclusion] All of the four antibiotics can be used to obtain dried Auricularia fruiting and temperature 65℃ can be used to isolate its tissue. After drying the fresh fruiting body without long time high temperature treatment, it can be separated successfully at room temperature for one year. Auricularia heimuer has the ability to germinate around the body
- Publication
Xinjiang Agricultural Sciences, 2021, Vol 58, Issue 4, p720
- ISSN
1001-4330
- Publication type
Article
- DOI
10.6048/j.issn.1001-4330.2021.04.015