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- Title
In Silico Identification of Dysregulated miRNAs Targeting KRAS Gene in Pancreatic Cancer.
- Authors
Garibaldi-Ríos, Asbiel Felipe; Figuera, Luis E.; Zúñiga-González, Guillermo Moisés; Gómez-Meda, Belinda Claudia; García-Verdín, Patricia Montserrat; Carrillo-Dávila, Irving Alejandro; Gutiérrez-Hurtado, Itzae Adonai; Torres-Mendoza, Blanca Miriam; Gallegos-Arreola, Martha Patricia
- Abstract
Pancreatic cancer (PC) is highly lethal, with KRAS mutations in up to 95% of cases. miRNAs inversely correlate with KRAS expression, indicating potential as biomarkers. This study identified miRNAs targeting KRAS and their impact on PC characteristics using in silico methods. dbDEMC identified dysregulated miRNAs in PC; TargetScan, miRDB, and PolymiRTS 3.0 identified miRNAs specific for the KRAS gene; and OncomiR evaluated the association of miRNAs with clinical characteristics and survival in PC. The correlation between miRNAs and KRAS was analysed using ENCORI/starBase. A total of 210 deregulated miRNAs were identified in PC (116 overexpressed and 94 underexpressed). In total, 16 of them were involved in the regulation of KRAS expression and 9 of these (hsa-miR-222-3p, hsa-miR-30a-5p, hsa-miR-30b-5p, hsa-miR-30e-5p, hsa-miR-377-3p, hsa-miR-495-3p, hsa-miR-654-3p, hsa-miR-877-5p and hsa-miR-885-5p) were associated with the clinical characteristics of the PC. Specifically, the overexpression of hsa-miR-30a-5p was associated with PC mortality, and hsa-miR-30b-5p, hsa-miR-377-3p, hsa-miR-495-3p, and hsa-miR-885-5p were associated with survival. Correlation analysis revealed that the expression of 10 miRNAs is correlated with KRAS expression. The dysregulated miRNAs identified in PC may regulate KRAS and some are associated with clinically relevant features, highlighting their potential as biomarkers and therapeutic targets in PC treatment. However, experimental validation is required for confirmation.
- Subjects
GENETIC regulation; GENE expression; RAS oncogenes; CANCER genes; PANCREATIC cancer
- Publication
Diseases, 2024, Vol 12, Issue 7, p152
- ISSN
2079-9721
- Publication type
Article
- DOI
10.3390/diseases12070152