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- Title
PROBIOTIC BACTERIA PROTECT LIPOPROTEINS FROM OXIDATION AND GLYCATION IN HYPERLIPIDEMICHYPERGLYCEMIC-HAMSTERS.
- Authors
Sorina Stancu, Camelia; Maria Sanda, Gabriela; Rogoz, Daniela; Volumnia Sima, Anca
- Abstract
Hyperlipidemia and diabetes are known to be associated with an increased oxidative stress and generation of glycation products which induce lipoproteins (Lp) modification. Lp oxidation or glycation make them aggressive for the cells of the arterial wall, accelerating the atherosclerosis process. Numerous antioxidant therapies were described, but no efficacy in regressing or arresting atherosclerosis and diabetes was observed in humans. Our aim was to assess the effect of probiotic bacteria Lactobacillus acidophilus and Bifidobacterium infantis on the circulating Lp oxidation and/or glycation. We used an animal model of fat diet-induced hyperlipidemia, which in time develops hyperglycemia, the hyperlipidemic-hyperglycaemic hamster. Male Golden Syrian hamsters fed standard chow supplemented with 3% cholesterol and 15% butter for 16 weeks were treated with a probiotic compound containing Lactobacillus acidophilus and Bifidobacterium infantis by gavage (P group) or received water by gavage (H group) for 5 weeks. A group receiving standard diet was used as control (N). Plasma Lp were separated by FPLC or ultracentrifugation and their oxidative status was evaluated spectrofluorimetrically. Paraoxonase 1 (PON1) activity was also measured. After 21 weeks of fat diet a significant increase of oxidized and glycated Lp levels was observed in plasma of H compared to N animals. Administration of the probiotic induced a decrease of the lipid oxidation products such as lipid peroxides and 4-hydroxynonenal (4-HNE) in all Lp: very low density lipoproteins (VLDL), low density lipoproteins (LDL) and high density lipoproteins (HDL). A significant reduction of the irreversibly glycated products as pentosidine, furoyl furanyl imidazole (FFI) or advanced glycated end-products (AGE) in all circulating Lp were determined. The decreased level of 4-HNE in HDL from P group was confirmed by western blot analysis and was associated with an increase in plasma PON1 activity, as compared to H group. In conclusion, probiotic bacteria Lactobacillus acidophilus and Bifidobacterium infantis exert anti-oxidative effects on circulating Lp and protect them from irreversible glycation. The results support the administration of probiotic bacteria as a possible therapy for prevention or reduction of atherosclerosis-associated diabetes by diminishing the pro-atherogenic properties of VLDL and LDL and potentiating the anti-atherogenic properties of HDL.
- Subjects
PROBIOTICS; LIPOPROTEINS; OXIDATION; PHYSIOLOGICAL effects of lipids; HAMSTERS; HYPERLIPIDEMIA; BIFIDOBACTERIUM; ATHEROSCLEROSIS; PHYSIOLOGY
- Publication
Annals of the Romanian Society for Cell Biology, 2012, Vol 17, Issue 2, p16
- ISSN
2067-3019
- Publication type
Article