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- Title
ETHANOL INDUCED HEPATIC STEATOSIS - MORPHO - FUNCTIONAL ASPECTS.
- Authors
Tutunaru, Dana; Ciocoiu, Manuela; Coman, Malina; Musat, Carmina; Nechita, A.; Badescu, Magda
- Abstract
In this study, we obtained an experimental model to assess potential toxic effects of alcohol, with the beneficial effects of alcohol association with soluble salts of zinc or vegetal polyphenols. The research was conducted on white Wistar mice, divided into four groups of 15 animals each: Group M - control group, Group A - animals which were treated with alcohol, Group A + Zn - animals which were treated with alcohol and Zn and Group A + P - animals which were treated with ethanol and polyphenols. Samples of blood were obtained and animal's death was induced after anesthesia with ether. Biochemical determinations were made in blood and tissular homogenate from liver at 1:10 rate of malondialdehyde value, as a nonspecific indicator of lipid peroxidation. Histological preparations were made from fragments of liver stained by the hematoxylin-eosin method for an overview of liver tissue, red Scharlach for triglyceride, red oil (O-Red) for lipids, van Gieson , PAS and trichromic Masson. Sections were examined with optical microscope at x 10, 20 and 40 zoom. For electronic microscopy, all specimens were fixed in glutaraldehyde and pressed with OsO4. It can be observed that both zinc and polyphenols decreased significantly (p <0.001) the amplitude of lipid peroxidation (reduction of malondialdehyde values) in serum and hepatocytes. Although the action of polyphenols is more intense, the difference between zinc effect and polyphenols effect is statistically insignificant. Photon microscopy examination of ethanol liver makes evident the followings: Hepatic steatosis; Zinc salts protects against lipidoperoxidation and polyphenols against collagen synthesis.
- Subjects
ALCOHOL research; FATTY degeneration; ETHANOL; ZINC salts; POLYPHENOLS; IN vivo toxicity testing; LABORATORY mice; MALONDIALDEHYDE
- Publication
Annals of the Romanian Society for Cell Biology, 2010, Vol 15, Issue 2, p362
- ISSN
2067-3019
- Publication type
Article