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- Title
木醋液对几种植物病原真菌的抑菌活性与作用机制.
- Authors
李彦湘; 丁德东; 何静; 张金花; 赵吉桃; 赵倩; 候彩霞; 朱珠
- Abstract
To explore the practical application of wood vinegar in the biological control of plant diseases and its mechanism of action against pathogenic fungi, experiments were conducted to evaluate the antimicrobial activity of wood vinegar against several tested plant pathogenic fungi by measuring the colony growth diameter and half effective concentration (EC50). The impact of wood vinegar on spore germination, mycelial dry weight, cell membrane permeability, cell wall integrity, soluble protein content, soluble sugar content, reducing sugar content, and adenosine triphosphate (ATP) content of sensitive fungal strains was assessed, and the ultrastructural changes of the fungal bodies were observed through electron microscopy to reveal its mechanism of action. The results showed that wood vinegar had certain inhibitory effects on all 7 tested plant pathogenic fungi, with the most significant inhibitory effect observed against Fusarium culmorum, with an EC50 value of 4.98 μL·mL-1, and the inhibition of spore germination and biomass at a volume fraction of 5.5 μL·mL-1 reached 90.09% and 95.22%, respectively. After treatment with wood vinegar, the cell structure of F. culmorum was damaged, the cell walls of the mycelium became thinner and incomplete, organelle structure was disrupted, and cell membrane permeability increased. Alkaline phosphatase activity, soluble sugar content, reducing sugar content, soluble protein content and ATP content of the mycelium were significantly reduced. Additionally, the fluorescence intensity of iodine-propidium iodide stained spores increased significantly. Wood vinegar mainly achieves its antimicrobial effect by damaging the fungal cell structure and interfering with fungal substance metabolism and energy metabolism.
- Publication
Acta Agriculturae Zhejiangensis, 2023, Vol 35, Issue 9, p2149
- ISSN
1004-1524
- Publication type
Article
- DOI
10.3969/j.issn.1004-1524.20221348