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- Title
华东山茶花腐病病原菌分离鉴定.
- Authors
普梅英; 武自强; 张诗文; 李艳杰; 朱幼娇; 吴坤; 陈龙清; 王超
- Abstract
The ornamental effect of Camellia japonica petal blight disease was very serious. In order to identify the pathogenic bacteria and biological characteristics of C. japonica petal blight disease and improve the quality and ornamental value of C. japonica, and provide the basis for prevention and control. The pathogen of petal blight disease of C. japonica from Kunming Botanical Garden in Kunming City was isolated by tissue separation method, and pathogenic bacteria were identified by pathogenicity assay, morphological and molecular biological identification. One strain was isolated from the C. japonica ‘Wucai’ diseased tissue, there was white hyphae on PDA medium and had black conidia in the later stage of culturing for 7 days. when it was connected back to the healthy petals, the disease of lacing petals after inoculation was more obvious, which could cause the flowers to become yellow and brown. When it was serious, it could make the petals dry rot or wet rot, and grew mycelium, which was consistent with the symptoms in the field and conformed to the pathogenicity determination of Koch’s rule. Based on the phylogenetic analysis of multiple primers (ITS, LSU, β-tubulin), Pestalotiopsis portugallica clustered in the same clade. It was confirmed that the pathogen of petal blight disease of C. japonica was P. portugallica. This was the first report that P. portugallica infected the flowers of C. japonica and caused petal blight disease.
- Subjects
KUNMING (China); CAMELLIAS; BOTANICAL gardens; GARDEN cities; PESTALOTIOPSIS; CONIDIA; PATHOGENIC bacteria
- Publication
Acta Agriculturae Zhejiangensis, 2023, Vol 35, Issue 1, p121
- ISSN
1004-1524
- Publication type
Article
- DOI
10.3969/j.issn.1004-1524.2023.01.13