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- Title
三 基于ITS2 和SNP技术鉴定浙江铁皮石斛的初步研究.
- Authors
陈文强; 汪小福; 陈笑芸; 彭 城; 徐俊锋; 蔡健
- Abstract
Dendrobium officinale is favored by consumers for its nutritional value and health benefits. However, it is difficult to distinguish and identify different varieties and different qualities of D. officinale. It is urgent to establish a fast, accurate and efficient identification method. In this study, 12 D. ofjicinale from Zhejiang Province and 10 other Dendrobium were used, the DNA barcode ITS2 was selected, the amplification system for ITS2 was optimized, then the amplified products were sequenced. After sequence comparison and analysis, the specific SNP sites were obtained from DNA barcode ITS2 of D. officinale; specific primers designed for the SNP sites were verified by PCR, and a rapid identification method for SNP sites based on high-resolution melting curve ( HRM) technology was established. The res ults showed that DNA barcode ITS2 could distinguish D. officinale from other Dendrobium. The rapid detection based on SNP realized the rapid identification of D. ofjicinale, which provided a new concept and technical path for identification and quality control of D. ofjicinale .
- Publication
Acta Agriculturae Zhejiangensis, 2021, Vol 33, Issue 1, p69
- ISSN
1004-1524
- Publication type
Article
- DOI
10.3969/j.issn.1004-1524.2021.01.09