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- Title
Expression and crystallization of a bacterial glycoside hydrolase family 116 β-glucosidase from Thermoanaerobacterium xylanolyticum.
- Authors
Sansenya, Sompong; Mutoh, Risa; Charoenwattanasatien, Ratana; Kurisu, Genji; Ketudat Cairns, James R.
- Abstract
The Thermoanaerobacterium xylanolyticum gene product TxGH116, a glycoside hydrolase family 116 protein of 806 amino-acid residues sharing 37% amino-acid sequence identity over 783 residues with human glucosylceramidase 2 (GBA2), was expressed in Escherichia coli. Purification by heating, immobilized metal-affinity and size-exclusion chromatography produced >90% pure TxGH116 protein with an apparent molecular mass of 90 kDa on SDS-PAGE. The purified TxGH116 enzyme hydrolyzed the p-nitrophenyl ( pNP) glycosides pNP-β-D-glucoside, pNP-β-D-galactoside and pNP- N-acetyl-β-D-glucopyranoside, as well as cellobiose and cellotriose. The TxGH116 protein was crystallized using a precipitant consisting of 0.6 M sodium citrate tribasic, 0.1 M Tris-HCl pH 7.0 by vapour diffusion with micro-seeding to form crystals with maximum dimensions of 120 × 25 × 5 µm. The TxGH116 crystals diffracted X-rays to 3.15 Å resolution and belonged to the monoclinic space group P21. Structure solution will allow a structural explanation of the effects of human GBA2 mutations.
- Subjects
HYDROLASES; GENE expression; GLYCOSIDASES; CRYSTALLIZATION; AMINO acid analysis; BACTERIAL enzymes
- Publication
Acta Crystallographica: Section F, Structural Biology Communications, 2015, Vol 71, Issue 1, p41
- ISSN
2053-230X
- Publication type
Article
- DOI
10.1107/S2053230X14025461