We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Analysis of the relationship between luminescence and toxicity of Vibrio carchariae pathogenic to shrimp.
- Authors
Nakayama, Tatsuya; Nomura, Nakao; Matsumura, Masatoshi
- Abstract
Aquaculture deterioration is caused by Vibrio harveyi, which is one of the main shrimp pathogens. Vibrio carchariae having the same phenotypic characteristics as V. harveyi was isolated from a shrimp farm and used in this study to investigate the relationship between luminescence and toxicity. Luminescence was verified by monitoring the expression of the LuxR gene. It was found that the relationship between the expression of LuxR and toxicity was the most significant factor related to luminescence. Results showed that LuxR expression was highest after 20 h of culture, which is comparable to the highest scintillation counter luminescence value (20 h). However, toxin levels in the culture broth were highest between 24 h-36 h of culture. Significant decrease in the toxicity level was observed after 36 h. It was also found that there was a decrease in toxicity level after 12 h of incubation at room temperature of the V. carchariae culture supernatant. The effect of the addition of cell-free culture supernatant on the luminescence of V. carchariae was also determined. Results showed that the addition of cell-free supernatant from 24 h-old culture was most effective in inducing and maintaining luminescence.
- Subjects
VIBRIO; LUMINESCENCE; PATHOGENIC microorganisms; SHRIMPS; EUBACTERIALES; AQUACULTURE; PATHOGENIC bacteria; SHRIMP fisheries
- Publication
Fisheries Science, 2005, Vol 71, Issue 6, p1236
- ISSN
0919-9268
- Publication type
Article
- DOI
10.1111/j.1444-2906.2005.01088.x