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- Title
Methylation Status of MTHFR Promoter and Oligozoospermia Risk: An Epigenetic Study and in Silico Analysis.
- Authors
Rezaeian, Atefeh; Karimian, Mohammad; Colagar, Abasalt Hossienzadeh
- Abstract
Objective: In this study, we evaluated the effects of promoter methylation of MTHFR on oligozoospermia risk, followed by an in silico analysis. Materials and Methods: In a case-control study, semen samples were collected from infertile and healthy control men. MTHFR promoter region was amplified by methylation-specific polymerase chain reaction (PCR). Finally, the promoter region of MTHFR was analyzed by bioinformatics software. Results: Our data revealed significant associations of CpG island promoter methylation with oligozoospermia in a case-control study. In silico analysis showed that promoter contains a strong nucleosome exclusion region, a bonafide CGIs, six PROSITE motifs without a defined TATA box and 14 transcription factor (TF) binding sites, which are directly involved in spermatogenesis Conclusion: Based on our findings, methylation of the MTHFR gene promoter region may be a risk factor for oligozoospermia. However, this is a preliminary report representing data for future comprehensive studies to make a clinical conclusion on the potential biomarker role of methylation of this promoter in elevating susceptibility to oligozoospermia.
- Subjects
METHYLATION; OLIGOSPERMIA; BIOINFORMATICS software; POLYMERASE chain reaction; PROMOTERS (Genetics); EPIGENETICS; CHROMATIN
- Publication
Cell Journal (Yakhteh), 2021, Vol 22, Issue 4, p482
- ISSN
2228-5806
- Publication type
Article
- DOI
10.22074/cellj.2021.6498