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- Title
Total bacterial populations in three lentic water bodies of the Colombian Andes using the epifluorescence technique.
- Authors
Canosa, Amparo; Pinilla, Gabriel
- Abstract
Abstract The epifluorescence technique was used to quantify the total bacterial population in two dams (Neusa and Prado) and in a natural lagoon (Fúquene), which have different geographical locations and physicochemical characteristics. Bacterial abundance was similar in the three water-bodies (8.4 × 108 cells L–1), but the density of plateable heterotrophs was always lower (1.4 × 106 CFU L–1) when obtained by the plate count method. Results obtained during validation of the epifluorescence technique show: (i) that there are no statistically significant differences between counts of samples fixed in the field and unfixed samples; (ii) that the median count of samples fixed and observed periodically over a period of 8 months was similar; (iii) that plate counts underestimate the population; and (iv) that epifluorescence counts were similar in the three water-bodies studied, despite their different ecological conditions.
- Subjects
ANDES Region; BACTERIAL pollution of water; PLATE counts (Microbiology); DIRECT epifluorescent filter technique; AQUATIC microbiology
- Publication
Lakes & Reservoirs: Research & Management, 2001, Vol 6, Issue 2, p169
- ISSN
1320-5331
- Publication type
Article
- DOI
10.1046/j.1440-1770.2001.00140.x