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- Title
Phenotypes Associated with Second Chromosome P Element Insertions in Drosophila melanogaster.
- Authors
Kahsai, Lily; Millburn, Gillian H.; Cook, Kevin R.
- Abstract
In Drosophila melanogaster, P element transposition has been a productive means of insertional mutagenesis. Thousands of genes have been tagged with natural and engineered P element constructs. Nevertheless, chromosomes carrying P element insertions tend to have high levels of background mutations from P elements inserting and excising during transposition. Consequently, the phenotypes seen when P element-bearing chromosomes are homozygous are often not attributable to the P insertions themselves. In this study, 178 strains in the Bloomington Drosophila Stock Center collection with P insertions on the second chromosome were complementation tested against molecularly defined chromosomal deletions and previously characterized single-gene mutations to determine if recessive lethality or sterility is associated with the P insertions rather than background mutations. This information should prove valuable to geneticists using these strains for experimental studies of gene function.
- Subjects
DROSOPHILA melanogaster; MUTAGENESIS; CHROMOSOMES
- Publication
G3: Genes | Genomes | Genetics, 2016, Vol 6, Issue 8, p2665
- ISSN
2160-1836
- Publication type
Article
- DOI
10.1534/g3.116.030940