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- Title
Experimentally Induced Psoriatic Lesions Associate with Rapid but Transient Decrease in Interleukin-33 Immunostaining in Epidermis.
- Authors
SUTTLE, Mireille-Maria; ENOKSSON, Mattias; ZOLTOWSKA, Anna; CHATTERJEE, Madhumita; NILSSON, Gunnar; HARVIMA, Ilkka T.
- Abstract
A slight epidermal damage can induce the Köbner reaction in psoriasis, and the "alarmin", interleukin-33 (IL-33), may be involved in this process. Therefore, the uninvolved psoriatic skin was tape-stripped, and skin biopsies were collected at 0 day, 2 h and 3 days or at 0 day, 1 day and 7 days for immunohistochemistry. Eight patients out of 18 with the positive Köbner reaction showed a decrease in epidermal thickness and revealed transient reduction in epidermal nuclear immunostaining of IL-33 in 2-h, 1-day, 3-day biopsies compared to the 10 Köbner-negative patients. In keratinocyte cultures, the full-length 32-kDa IL-33 was detected after damaging the cells with freeze-thawing. Interestingly, a very low concentration of rh-IL-33 (0.001-0.01 ng/ml) significantly stimulated ³H-thymidine uptake by human LAD2 mast cells, but not by psoriatic peripheral blood mononuclear cells. The results show that epidermal IL-33 associates with positive Köbner response, and only a small amount of the IL-33 apparently released may induce proliferation in dermal mast cells.
- Subjects
PSORIASIS; INTERLEUKINS; IMMUNOSTAINING; MAST cells; GROWTH factors
- Publication
Acta Dermato-Venereologica, 2015, Vol 95, Issue 5, p536
- ISSN
0001-5555
- Publication type
Article
- DOI
10.2340/00015555-2018