We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
GTP-dependent run-up of Piezo2-type mechanically activated currents in rat dorsal root ganglion neurons.
- Authors
Zhanfeng Jia; Ikeda, Ryo; Jennifer Ling; Jianguo G. Gu
- Abstract
Rapidly adapting mechanically activated channels (RA) are expressed in primary afferent neurons and identified as Piezo2 ion channels. We made whole-cell voltage-clamp recordings from cultured dorsal root ganglion (DRG) neurons to study RA channel regulation. RA currents showed gradual increases in current amplitude (current "run-up") after establishing whole-cell mode when 0.33 mM GTP or 0.33 mM GTPγS was included in the patch pipette internal solution. RA current run-up was also observed in HEK293 cells that heterologously expressed Piezo2 ion channels. No significant RA current run-up was observed in DRG neurons when GTP was omitted from the patch pipette internal solution, when GTP was replaced with 0.33 mM GDP, or when recordings were made under the perforated patchclamp recording configuration. Our findings revealed a GTP-dependent up-regulation of the function of piezo2 ion channels in DRG neurons.
- Subjects
GANGLIA; NEURONS; CELLS; ION channels; PIPETTES
- Publication
Molecular Brain, 2013, Vol 6, Issue 1, p2
- ISSN
1756-6606
- Publication type
Article
- DOI
10.1186/1756-6606-6-57