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- Title
Genome‐resolved metagenomics identifies novel active microbes in biogeochemical cycling within methanol‐enriched soil.
- Authors
Macey, Michael C.
- Abstract
Metagenome assembled genomes (MAGs), generated from sequenced 13C‐labelled DNA from 13C‐methanol enriched soils, were binned using an ensemble approach. This method produced a significantly larger number of higher‐quality MAGs compared to direct binning approaches. These MAGs represent both the primary methanol utilizers and the secondary utilizers labelled via cross‐feeding and predation on the labelled methylotrophs, including numerous uncultivated taxa. Analysis of these MAGs enabled the identification of multiple metabolic pathways within these active taxa that have climatic relevance relating to nitrogen, sulfur and trace gas metabolism. This includes denitrification, dissimilatory nitrate reduction to ammonium, ammonia oxidation and metabolism of organic sulfur species. The binning of viral sequence data also yielded extensive viral MAGs, identifying active viral replication by both lytic and lysogenic phages within the methanol‐enriched soils. These MAGs represent a valuable resource for characterizing biogeochemical cycling within terrestrial environments.
- Subjects
BIOGEOCHEMICAL cycles; METAGENOMICS; SULFUR metabolism; DENITRIFICATION; TRACE gases
- Publication
Environmental Microbiology Reports, 2024, Vol 16, Issue 2, p1
- ISSN
1758-2229
- Publication type
Article
- DOI
10.1111/1758-2229.13246