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- Title
DEVELOPMENT AND VALIDATION OF SPECTROFLUOROMETRIC METHOD FOR DETERMINATION OF PIOGLITAZONE HCL IN MARKETED AND NON-MARKETED PHARMACEUTICAL TABLET DOSAGE FORMS.
- Authors
ALYAMI, HAMAD S.; ABU ALRUB, SAMER S.; MAHNASHI, MATER H.; ALHARTHI, ABDULLAH N.
- Abstract
Pioglitazone hydrochloride is commonly used for the management of type-II diabetes. It is analyzed by various methods based on chromatographic techniques which are time and resource consuming. The objective of this study was to develop a simple, rapid, accurate, and cost-effective method for the analysis of pioglitazone hydrochloride in bulk and pharmaceutical dosage forms. In the present study, various experimental conditions were optimized according to USP and ICH guidelines. A solvent was selected based on the solubility while working wavelengths were selected based on the scan of a 1% solution, prepared in a selected solvent. Applicability of the method was assessed by the comparison of results with the developed method and methods reported for pioglitazone hydrochloride. The fluorescence intensity was measured in water/methanol solvent at 521 and at 260 nm for pioglitazone HCl excitation. Beer's law was obeyed in the concentration range of 0.02-0.06 µg/mL, with a good correlation coefficient of R2, equal to 0.9613 between fluorescence intensity and concentration. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.0102 and 0.0309 µg/mL, respectively. The method is applicable for the determination of pioglitazone HCl in different dosage forms with an average recovery of 85.36 to103.0%.
- Subjects
FLUORIMETRY; PIOGLITAZONE; WAVELENGTHS; DRUGS; CHROMATOGRAPHIC analysis
- Publication
Acta Poloniae Pharmaceutica, 2020, Vol 77, Issue 5, p699
- ISSN
0001-6837
- Publication type
Article
- DOI
10.32383/appdr/128376