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- Title
Hemolysis of red blood cells during processing and storage.
- Authors
Gkoumassi, Effimia; Dijkstra-Tiekstra, Margriet J.; Hoentjen, Dinie; de Wildt-Eggen, Janny
- Abstract
BACKGROUND: During processing and storage, red blood cells (RBCs) undergo changes and cell injury resulting in hemolysis. Mostly, the separation of whole blood in top-and-bottom quadruple bag systems with break openings takes less than 4 minutes. However, longer separation times are not uncommon. The aims were to investigate whether hemolysis is increased in RBCs with longer separation time (RBCs>6 min) compared to regular RBCs (RBCsreg), to measure hemolysis increase during storage and to study whether frequency of hemolytic donations is donor dependent. STUDY DESIGN AND METHODS: RBCs>6 min (n = 172) and 172 matched controls were tested for hemolysis on Days 1 and 21 RBC units from each group were stored at 4 ± 2°C and tested again after 5 weeks. Donor dependency was retrospectively investigated for 100 hemolytic RBC units. RESULTS: RBCs>6 min exhibited a higher mean hemolysis rate than RBCsreg (0.058% vs. 0.033%). Four RBC units were hemolytic (>0.8%), all RBCs>6 min (2.36%). During storage, hemolysis in both groups increased with 0.24%. Hemolysis frequency did not seem to be donor dependent. CONCLUSIONS: Increased separation time is a useful screening tool for potentially increased hemolysis rate in RBCs. Hemolysis rate increased during storage equally in both groups. Hemolysis frequency appears donor independent.
- Subjects
ERYTHROCYTES; HEMOLYSIS &; hemolysins; BUFFY coat; HEMOGLOBIN synthesis; UNITED States. Food &; Drug Administration
- Publication
Transfusion, 2012, Vol 52, Issue 3, p489
- ISSN
0041-1132
- Publication type
Article
- DOI
10.1111/j.1537-2995.2011.03298.x