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- Title
Assessment of Vitamin A versus Vitamin E effect on Motility and DNA Integrity of Human Cryopreserved Sperms.
- Authors
Al-Anbari, Samir A.; Ibraheem, Mustafa M.; Farhan, Thaer M.
- Abstract
Background: It is well known that cryopreservation procedure may result in negative impact on spermatozoa function throughout relative overproduction of reactive oxygen species (ROS). However, ROS overproduction can be corrected by antioxidants. Objective: To find out the effect of vitamin A on cryopreserved spermatozoa throughout measuring their motility rate and DNA fragmentation and compare it with vitamin E effect. Methods: Forty seminal fluid specimens were individually collected from forty healthy, non-drug and nonalcohol consumers, normozoospermic males in Um Al-Baneen Center for Infertility Management and In Vitro Fertilization in Baghdad. To facilitate comparing between used vitamins and experimenting each one alone, those specimens were distributed into 4 groups of 10 specimens each. Following deriving their own controls without any vitamin treatment, specimens of these groups, in general, were treated with two concentrations of vitamin A (20 μg/dl and 30 μg/dl), and two concentrations of vitamin E (10 μmol/l and 20 μmol/l). Then, each specimen was incubated for 1 hour before being cryopreserved in liquid nitrogen for 14 days. Motility percentage and DNA fragmentation were assessed following cryopreservation and thawing of spermatozoa. Results: Results revealed that there were significant statistically differences in post-thawing motility and DNA fragmentation means between specimens treated with vitamin A and their relevant control; between specimens treated with vitamin E and their relevant control; and between specimens treated with vitamin E and those treated with vitamin A among all groups of the study. Conclusion: These results lead to the conclusion that both of vitamin A and E play an important role in improving and protecting sperm motility and DNA integrity following cryopreservation, and vitamin E is more effective than vitamin A.
- Subjects
BAGHDAD (Iraq); VITAMIN A; VITAMIN E; CRYOPRESERVATION of cells; HUMAN DNA; REACTIVE oxygen species; SPERMATOZOA
- Publication
Iraqi Journal of Medical Sciences, 2023, Vol 21, Issue 1, p43
- ISSN
1681-6579
- Publication type
Article
- DOI
10.22578/IJMS.21.1.5