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- Title
Rapid DNA-genotyping system targeting ten loci for resistance to blast disease in rice.
- Authors
Noriyuki Kitazawa; Ayahiko Shoniura; Tatsumi Mizubavashi; Tsuyu Ando; Kazufumi Nagata; Nagao Hayashi; Akira Takahashi; Utako Yamanouchi; Shuichi Fukuoka
- Abstract
The fungal pathogen Pyricularia ory'zae causes blast, a severe disease of rice (Oryza sativa L.). Improving blast resistance is important in rice breeding programs. Inoculation tests have been used to select for resistance genotypes, with DNA marker-based selection becoming an efficient alternative. No comprehensive DNA marker system for race-specific resistance alleles in the Japanese rice breeding program has been developed because some loci contain multiple resistance alleles. Mere, we used the Fluidigm SNP genotyping platform to determine a set of 96 single nucleotide polymorphism (SNP) markers for 10 loci with race-specific resistance. The markers were then used to evaluate the presence or absence of 24 resistance alleles in 369 cultivars; results were 93.5%consistent with reported inoculation test-based genotypes in japonica varieties. The evaluation system was successfully applied to high-yield varieties with indica genetic backgrounds. The system includes polymorphisms that distinguish the resistant alleles at the tightly linked Pita and Pita-2 loci, thereby confirming that all the tested cultivars with Pita-2 allele carry Pita allele. We also developed and validated insertion/ deletion (InDel) markers for ten resistance loci. Combining SNP and InDel markers is an accurate and efficient strategy for selection for race-specific resistance to blast in breeding programs.
- Subjects
RICE blast disease; DISEASE resistance of plants; SINGLE nucleotide polymorphisms; RICE breeding; RICE
- Publication
Breeding Science, 2019, Vol 69, Issue 1, p68
- ISSN
1344-7610
- Publication type
Article
- DOI
10.1270/jsbbs.18143