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- Title
De novo assembly of the complete organelle genome sequences of azuki bean (Vigna angularis) using next-generation sequencers.
- Authors
Ken Naito; Akito Kaga; Norihiko Tomooka; Makoto Kawase
- Abstract
sequences of those organelle genomes have been broadly used in phylogenetic studies. Thanks to recent progress in next-generation sequencer (NGS) technology, whole-genome sequencing can be easily performed. Here, using NGS data generated by Roche GS Titanium and Illumina Hiseq 2000, we performed a hybrid assembly of organelle genome sequences of Vigna angularis (azuki bean). Both the mitochondrial genome (mtDNA) and the chloroplast genome (cpDNA) of V. angularis have very similar size and gene content to those of V. radiata (mungbean). However, in structure, mtDNA sequences have undergone many recombination events after divergence from the common ancestor of V. angularis and V. radiata, whereas cpDNAs are almost identical between the two. The stability of cpDNAs and the variability of mtDNAs was further confirmed by comparative analysis of Vigna organelles with model plants Lotus japónicas and Arabidopsis thaliana.
- Subjects
AZUKI; PLANT organelles; NUCLEOTIDE sequence; PHYLOGENY; CHLOROPLAST DNA; MITOCHONDRIAL DNA
- Publication
Breeding Science, 2013, Vol 63, Issue 2, p176
- ISSN
1344-7610
- Publication type
Article
- DOI
10.1270/jsbbs.63.176