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- Title
The Relationship of Pronuclear Stage Morphology and Chromosome Status at Cleavage Stage.
- Authors
Chun-Kai Chen; Guan-Yi Shen, Larry I.; Shang-Gwo Horng; Chia-Woei Wang; Hong-Yuan Huang; Hsin-Shin Wang, Larry I.; Yung-Kuei Soong
- Abstract
Purpose: Infertile couples undergoing in vitro fertilization-embryo transfer (IVF-ET) program were included to study the relationship of pronuclear stage morphology and chromosome status at cleavage stage. Methods: Eighteen to twenty-one hours after fertilization, zygotes were checked for pronuclear morphology with modified Scott Z-score system. After embryo transfer on day 3, arrested or non-transferred 2 PN embryos were spread for fluorescence in sim hybridization (FISH) staining of probes to chromosomes 18, X and Y. Results: Ninety-eight embryos were successfully fixed and stained. The chromosome status were recorded in each 2 PN score group: 7 (54%) of 13 embryos in Z2 group, 14 (35%) of 40 in Z3 group and 10 (36%) of 28 in Z4 group being normal diploid. Z1 group has 12 (71%) of 17 embryos being normal diploid, which is significantly more than Z3 (p = 0.020) and Z4 group (p = 0.033). Conclusion: Our results demonstrated a high probability to get normal diploid embryos if good morphology at pronuclear stage was used as selection criteria, especially for Z1 score embryos.
- Subjects
CHROMOSOMES; EMBRYOS; EMBRYOLOGY; INFERTILITY
- Publication
Journal of Assisted Reproduction & Genetics, 2003, Vol 20, Issue 10, p413
- ISSN
1058-0468
- Publication type
Article
- DOI
10.1023/A:1026232625659