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- Title
Activation of AMPK inhibits Galectin-3-induced pulmonary artery smooth muscle cells proliferation by upregulating hippo signaling effector YAP.
- Authors
Zhang, Qianqian; Li, Wenge; Zhu, Yanting; Wang, Qingting; Zhai, Cui; Shi, Wenhua; Feng, Wei; Wang, Jian; Yan, Xin; Chai, Limin; Chen, Yuqian; Li, Cong; Liu, Pengtao; Li, Manxiang
- Abstract
Galectin-3(Gal-3) is an effective regulator in the pathological process of pulmonary arterial hypertension (PAH). However, the detailed mechanisms underlying Gal-3 contribution to PAH are not yet entirely clear. The aim of the present study was to explore these issues. Proliferation of rat pulmonary arterial smooth muscle cells (PASMCs) was determined using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Small interfering RNA (siRNA) was applied to silence the expression of yes-associated protein (YAP) and Forkhead box M1 (FOXM1). The protein expression and phosphorylation were measured by immunoblotting. The subcellular location of YAP was determined using immunoblotting and immunofluorescence. Gal-3-stimulated PASMCs proliferation in a time- and dose-dependent manner, this was accompanied with, YAP upregulation, dephosphorylation, and nucleus translocation. Gal-3 further increased FOXM1 and cyclinD1 expression via YAP activation. Interfering YAP/FOXM1 axis suppressed Gal-3-induced PASMCs proliferation. Activation of AMPK also inhibited Gal-3-triggered cells proliferation by targeting YAP/FOXM1/cyclinD1 pathway. Gal-3 induced PASMCs proliferation by regulating YAP/FOXM1/cyclinD1 signaling cascade, and activation of AMPK targeted on this axis and suppressed Gal-3-stimulated PASMCs proliferation. Our study provides novel therapeutic targets for prevention and treatment of PAH.
- Subjects
SMOOTH muscle; MUSCLE cells; INHIBITION of cellular proliferation; CELL proliferation; SMALL interfering RNA; VASCULAR smooth muscle
- Publication
Molecular & Cellular Biochemistry, 2021, Vol 476, Issue 8, p3037
- ISSN
0300-8177
- Publication type
Article
- DOI
10.1007/s11010-021-04131-3