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- Title
<italic>Streptomyces swartbergensis</italic> sp. nov., a novel tyrosinase and antibiotic producing actinobacterium.
- Authors
le Roes-Hill, Marilize; Prins, Alaric; Meyers, Paul R.
- Abstract
As part of an antibiotic screening program, an actinobacterium, strain HMC13T, was isolated from soil collected from the banks of the Gamka River, Western Cape Province, South Africa. The isolate was found to produce branched mycelia that differentiated into spiral spore chains with spiny spores. 16S rRNA gene sequence analysis showed the strain to be closely related to <italic>Streptomyces caelestis</italic> NRRL 2418T (99.72%) and <italic>Streptomyces azureus</italic> NBRC 12744T (99.51%). Chemotaxonomic analyses confirmed the classification of the strain as a member of the genus <italic>Streptomyces</italic>: LL-DAP in the peptidoglycan, no diagnostic sugars in the whole cell sugar pattern, dominant menaquinones including MK9(H8), MK9(H6), and the polar lipids detected included phosphatidylethanolamine. The fatty acid profile revealed the presence of mostly branched, saturated fatty acids: iso-C15:0 (14.4%), anteiso-C15:0 (21.1%), iso-C16:0 (16.8%), C16:1ω7<italic>c</italic>/2-OH iso-C15:0 (5.8%), C16:0 (6.2%), iso-C17:1ω9<italic>c</italic> (5.8%), iso-C17:0 (5.9%), and anteiso-C17:0 (9.6%). Strain HMC13T is a tyrosinase producer and exhibits very strong antibiosis against <italic>Mycobacterium aurum</italic> A+ and <italic>Staphylococcus aureus</italic> subsp. <italic>aureus</italic> ATCC 33591 (methicillin resistant), while only weak activity was observed against <italic>Bacillus cereus</italic> ATCC 10876, <italic>Enterococcus faecium</italic> VanA (vancomycin resistant), <italic>Enterococcus faecalis</italic> ATCC 51299 (vancomycin resistant) and <italic>Candida tropicalis</italic> ATCC 750T. Strain HMC13T (= LMG 28849T = NRRL B-65294T) is proposed as the type strain of a new species, to be named <italic>Streptomyces swartbergensis</italic> sp. nov.
- Subjects
STREPTOMYCES; PHENOL oxidase; ACTINOBACTERIA; SOIL microbiology; BACTERIAL spores
- Publication
Antonie van Leeuwenhoek, 2018, Vol 111, Issue 4, p589
- ISSN
0003-6072
- Publication type
Article
- DOI
10.1007/s10482-017-0979-3