We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Oligonucleotide primers for specific detection of actinobacterial laccases from superfamilies I and K.
- Authors
Fernandes, Tatiana; Silveira, Wendel; Passos, Flávia; Zucchi, Tiago
- Abstract
Although many putative laccase-like genes have been assigned to members of the phylum Actinobacteria, few of the related enzymes have been characterized so far. It is noteworthy, however, that this small number of enzymes has presented properties with industrial relevance. This observation, combined with the recognized biotechnological potential and the capability of this phylum to degrade recalcitrant soil polymers, has attracted attention for bioprospective approaches. In the present work, we have designed and tested primers that were specific for detection of sub-groups of laccase-like genes within actinomycetes, which corresponded to the superfamilies I and K from the classification presented by the laccase and multicopper oxidase engineering database. The designed primers have amplified laccase-like gene fragments from actinomycete isolates that were undetectable by primers available from the literature. Furthermore, phylogenetic alignments suggest that some of these fragments may belong to new laccases-like proteins, and thus emphasize the benefits of designing subgroup-specific primers.
- Subjects
NUCLEIC acid probes; DNA primers; LACCASE; ACTINOBACTERIA; ENGINEERING databases; BIOTECHNOLOGY; PHYLOGENY
- Publication
Antonie van Leeuwenhoek, 2014, Vol 106, Issue 2, p391
- ISSN
0003-6072
- Publication type
Article
- DOI
10.1007/s10482-014-0193-5