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- Title
Biological applications of microchip electrophoresis with amperometric detection: in vivo monitoring and cell analysis.
- Authors
Schilly, Kelci M.; Gunawardhana, Shamal M.; Wijesinghe, Manjula B.; Lunte, Susan M.
- Abstract
Microchip electrophoresis with amperometric detection (ME-EC) is a useful tool for the determination of redox active compounds in complex biological samples. In this review, a brief background on the principles of ME-EC is provided, including substrate types, electrode materials, and electrode configurations. Several different detection approaches are described, including dual-channel systems for dual-electrode detection and electrochemistry coupled with fluorescence and chemiluminescence. The application of ME-EC to the determination of catecholamines, adenosine and its metabolites, and reactive nitrogen and oxygen species in microdialysis samples and cell lysates is also detailed. Lastly, approaches for coupling of ME-EC with microdialysis sampling to create separation-based sensors that can be used for near real-time monitoring of drug metabolism and neurotransmitters in freely roaming animals are provided.
- Subjects
MICROCHIP electrophoresis; CELL analysis; REACTIVE oxygen species; COMPLEX compounds; DRUG monitoring; DRUG metabolism
- Publication
Analytical & Bioanalytical Chemistry, 2020, Vol 412, Issue 24, p6101
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-020-02647-z