We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Assessment of the Expression of IRβ, IRS-1, IRS-2 and IGF-IRβ in a Rat Model of Intrauterine Growth Restriction.
- Authors
Bueno, Marcia Pereira; Guadagnini, Dioze; Gonçalves, Frances Lilian Lanhellas; Barini, Ricardo; Saad, Mario José Abdalla; Schmidt, Augusto Frederico; Sbragia, Lourenço
- Abstract
Objective: To investigate glomerular development and expression of insulin and insulin-like growth factor receptors in an experimental model of intrauterine growth restriction (IUGR). Material and Methods: We studied three groups of Sprague-Dawley fetuses: IUGR - restricted by ligation of the right uterine artery; C-IUGR - left horn controls, and EC - external controls (non-manipulated). Body and organs were weighed, and glomerular number and volume were analyzed. Expression of IRβ, IRS-1, IRS-2 and IGF-IRβ was analyzed in liver, intestine and kidneys by immunoblotting. Results: Organ/body weight ratios were similar. In IUGR, glomerular number and volume were increased compared to C-IUGR and EC (p<0.001). In the IUGR liver, increases were found in IGF-IRβ compared to C-IUGR and EC; IRβ compared to EC, and IRS-2 compared to C-IUGR. However, decreases in IRβ were noted in IUGR compared to C-IUGR; IRS-1 compared to C-IUGR and EC, and IRS-2 compared to EC. In IUGR intestine, increases were detected in IRβ, IRS-1 and IGF-IRβ compared to C-IUGR and EC. In IUGR kidneys, increases were observed in IRβ and IGF-IRβ compared to C-IUGR and EC, and IRS-1 compared to EC. Decreased IRS-2 in the intestine and kidney were noticed in IUGR compared to C-IUGR and EC. Conclusion: IUGR fetuses had less glomeruli and alterations in insulin receptors, which may be associated with an increased risk of disease occurrence in adulthood. Copyright © 2010 S. Karger AG, Basel
- Subjects
FETAL growth disorders; INSULIN receptors; FETAL development; GENE expression; SOMATOMEDIN; IMMUNOBLOTTING; LABORATORY rats; GLOMERULAR filtration rate
- Publication
Fetal Diagnosis & Therapy, 2010, Vol 28, Issue 3, p145
- ISSN
1015-3837
- Publication type
Article
- DOI
10.1159/000316932