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- Title
Role of Aquaporin-5 in Gallbladder Carcinoma.
- Authors
Sekine, S.; Shimada, Y.; Nagata, T.; Sawada, S.; Yoshioka, I.; Matsui, K.; Moriyama, M.; Omura, T.; Osawa, S.; Shibuya, K.; Hashimoto, I.; Watanabe, T.; Hojo, S.; Hori, R.; Okumura, T.; Yoshida, T.; Tsukada, K.
- Abstract
Background/Purpose: Aquaporins (AQPs) are important in controlling bile formation. However, the exact role in human gallbladder carcinogenesis has not yet been defined. Methods: AQP-5-expressing gallbladder carcinoma (GBC) cell lines (NOZ) were transfected with anti-AQP-5 small interfering RNA (siRNA). Growth, migration, invasion assay, and drug susceptibility tests were performed. Next, microRNA (miRNA) expression was analyzed by miRNA oligo chip (3D-Gene®). AQP-5 and AQP-5-related miRNA target gene expressions were also analyzed using tissue microarray (TMA) in 44 GBC samples. Results: Treatment with AQP-5 siRNA decreased cell proliferation, migration, and invasion. On the other hand, those cells increased IC50 of gemcitabine. By performing miRNA assays, miR-29b, -200a, and -21 were shown to be highly overexpressed in cells treated with AQP-5 siRNA NOZ. When focusing on miR-21, phosphatase and tensin homolog (PTEN) was found to be a target of miR-21. In the TMA, AQP-5/PTEN coexpression was significantly associated with the depth of invasion and MIB-1 index (p = 0.003, 0.010). Survival of patients with a high AQP-5/PTEN coexpression was longer than that of patients with a low coexpression (p = 0.003). Conclusions: Our result suggested that miR-21 and PTEN may contribute to the role of AQP-5 in GBC. AQP-5 and PTEN cascades are favorable biomarkers of GBC. © 2013 S. Karger AG, Basel
- Subjects
GALLBLADDER cancer; AQUAPORINS; SMALL interfering RNA; PHOSPHATASES; IMMUNOHISTOCHEMISTRY; MICRORNA
- Publication
European Surgical Research, 2014, Vol 51, Issue 3/4, p108
- ISSN
0014-312X
- Publication type
Article
- DOI
10.1159/000355675