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- Title
Multilayer petal-like enzymatic-inorganic hybrid micro-spheres [CPO-(Cu/Co/Cd)3(PO4)2] with high bio-catalytic activity.
- Authors
Wang, Shengjie; Ding, Yu; Chen, Rui; Hu, Mancheng; Li, Shuni; Zhai, Quanguo; Jiang, Yucheng
- Abstract
Graphical abstract Highlights • Enzymatic-inorganic hybrid microsphere was prepared in aqueous solution. • The formation of multilayer structures are studied by various methods. • The hybrid materials shows excellent bio-catalytic activity, stability and reusability. Abstract CPO, a versatile enzyme, has limitations in industrial applications due to its poor stability and the difficulty of reuse. The preparation of stable immobilized enzymes with high catalytic activity is therefore desirable, but remains a challenge. A facile preparation of a series of enzyme-inorganic hybrid micro-spheres [chloroperoxidase (CPO)-(Cu/Co/Cd) 3 (PO 4 ) 2 ] and its application in the decolorization of crystal violet is reported in this work. All the hybrid micro-spheres show a multilayer petal-like structure. The formation of hybrid micro-spheres were proposed via four stages: crystallization, in-situ coordination, self-assembly and size growth. The process was entropy-driven, and there was a competition between precipitation of phosphate and coordination of M2+ with amide groups of CPO. The introduction of excess of chloride ions retarded the phosphate from precipitating by forming [MCl 4 ]2− complexes, while simultaneously promoting the coordination of M2+ with amide groups. These hybrid materials showed high bio-catalytic activity in the decolorization of crystal violet. The decolorization efficiency reached 99.66%, 98.44% and 98.05% just within 3 min using CPO-Cu 3 (PO 4 ) 2 , CPO-Cd 3 (PO 4 ) 2 and CPO-Co 3 (PO 4 ) 2 respectively. They also have good thermal stability, and can keep 52.89% catalytic activity after 8 cycles of use. CPO-inorganic hybrid micro-spheres [chloroperoxidase (CPO)-(Cu/Co/Cd) 3 (PO 4 ) 2 ] is promising in the treatment of waste water containing crystal violet. It also has potential application for treatment of other soluble organic dyes in industrial wastewater.
- Subjects
CHLOROPEROXIDASE; BIOCATALYSIS; AQUEOUS solutions; IMMOBILIZED enzymes; GENTIAN violet
- Publication
Chemical Engineering Research & Design: Transactions of the Institution of Chemical Engineers Part A, 2018, Vol 134, p52
- ISSN
0263-8762
- Publication type
Article
- DOI
10.1016/j.cherd.2018.03.036