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- Title
Effect of storage temperature and antibiotic impregnation on the quantity of bone morphogenetic protein seven in human bone grafts.
- Authors
Wurm, Alexander; Nogler, Michael; Ammann, Christoph; Coraça-Huber, Débora
- Abstract
Purpose: The aim of this study was to quantify the amount of bone morphogenic protein 7 (BMP-7) in bone samples in different storage and treatment conditions used in bone banks and thereby evaluate the benefit of this test as a routine measure before bone grafting. Methods: Fresh as well as frozen bone chips, each with and without antibiotic impregnation, were screened for their BMP-7 content. Human bone chips were produced from femoral heads of two female donors who had undergone total hip replacement surgery. The amount of BMP-7 was detected using a commercially available enzyme-linked immunosorbent assay (ELISA) test. Results: There were no significant differences between groups in samples obtained from the first femoral head. Bone-chip samples derived from the second femoral head showed significant differences between groups. The actual amount of these differences was small and most likely biologically irrelevant. It is important to note that there was a significant difference between groups when comparing both femoral heads, reflecting donor-to-donor variability. Conclusion: ELISA testing for BMP-7 as a qualitative measurement of bone grafts should be considered a routine quality-control test for bone banks.
- Subjects
BONE grafting; ANTIBIOTICS; BONE proteins; FEMUR; ENZYME-linked immunosorbent assay; BONE banks
- Publication
International Orthopaedics, 2014, Vol 38, Issue 7, p1513
- ISSN
0341-2695
- Publication type
Article
- DOI
10.1007/s00264-014-2349-3