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- Title
One Strain-Many Compounds Method for Production of Diverse Metabolic Profiles Using Chaetomium sp. from Astragalus membranaceus.
- Authors
Zhang, Hongchi; Liu, Rui; Yang, Yang; Li, Hui; Zhou, Feng
- Abstract
Subfraction B2-2 was by further subjected to repeated silica gel column chromatography and semipreparative reversed-phase HPLC (H SB 2 sb O-MeOH, 2:3) to afford compounds B 1 b and B 2 b . Subfraction B2 was separated by silica gel column chromatography and eluted with petroleum ether-ethyl acetate to give compound B 6 b and B 1 b . Subfraction B2-3 was by further repeated silica gel column chromatography and semipreparative RP-HPLC (H SB 2 sb O-MeOH, 3:2) to afford compound B 1 b . The culture afforded a dark brown crude extract, which was subjected to silica gel column chromatography and eluted successively with ethyl acetate-methanol gradient to yield six fractions (A-F).
- Subjects
ASTRAGALUS membranaceus; CHAETOMIUM; NORMAL-phase chromatography; PRODUCTION methods; MICROBIAL metabolites
- Publication
Chemistry of Natural Compounds, 2021, Vol 57, Issue 6, p1177
- ISSN
0009-3130
- Publication type
Article
- DOI
10.1007/s10600-021-03581-x