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- Title
Applications of a highly α2,6-selective pseudosialidase.
- Authors
Both, Peter; Riese, Michel; Gray, Christopher J.; Kun Huang; Pallister, Edward G.; Kosov, Iaroslav; Conway, Louis P.; Voglmeir, Josef; Flitsch, Sabine L.
- Abstract
Within human biology, combinations of regioisomeric motifs of α2,6- or α2,3-sialic acids linked to galactose are frequently observed attached to glycoconjugates. These include glycoproteins and glycolipids, with each linkage carrying distinct biological information and function. Microbial linkage-specific sialidases have become important tools for studying the role of these sialosides in complex biological settings, as well as being used as biocatalysts for glycoengineering. However, currently, there is no α2,6-specific sialidase available. This gap has been addressed herein by exploiting the ability of a Photobacterium sp. α2,6-sialyltransferase to catalyze trans-sialidation reversibly and in a highly linkage-specific manner, acting as a pseudosialidase in the presence of cytidine monophosphate. Selective, near quantitative removal of α2,6-linked sialic acids was achieved from a wide range of sialosides including small molecules conjugates, simple glycan, glycopeptide and finally complex glycoprotein including both linkages.
- Subjects
SIALIC acids; GALACTOSE; GLYCOLIPIDS; GLYCOCONJUGATES; PHOTOBACTERIUM
- Publication
Glycobiology, 2018, Vol 28, Issue 5, p261
- ISSN
0959-6658
- Publication type
Article
- DOI
10.1093/glycob/cwy016