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- Title
糜子 PmNAC1 的克隆及生物信息学分析.
- Authors
辛旭霞; 郑香然; 王海岗; 陈 凌; Santra Dipak K.; 王瑞云; 乔治军
- Abstract
Broomcorn millet is a gramineous herb with drought resistance, saline-alkali tolerance, barren tolerance, and short growth period. In order to provide candidate genes for stress resistance molecular breeding for broomcorn millet, in this study, on the basis of the previous transcriptome sequencing annotation genes, the NAC protein domain annotated on the chromosome 16 of the broomcorn millet genome was aligned to obtain the gDNA sequence of the PmNAC1 gene. The nucleotide sequence of the broomcorn millet was obtained by PCR amplification and gene cloning. The online software ORF Finder Blast were used to conduct bioinformatics analysis of the physical and chemical properties and structure of the protein encoded by broomcorn millet NAC. The results showed that the full length of the PmNAC1 gene was 1 634 bp, encoding 423 amino acids, without signal peptide and transmembrane structure. The amino acids in the secondary structure were mainly composed of 8 α-helix chains and 14 β-sheet chains, which were hydrophilic proteins. The conserved sites were 73-211 amino acids, and there were 59 phosphorylation sites. The glycosylation site exceeded the horizontal line of 0.5. The phylogenetic tree of NAC amino acid sequence of broomcorn millet transcription factor was constructed. The genetic relationship between PmNAC1 protein and switchgrass CUC2 was the closest, and the genetic variation coefficient of the two was 0.06.
- Subjects
BROOMCORN millet; IMINO acids; AMINO acid sequence; ANALYTICAL chemistry; MOLECULAR cloning; SWITCHGRASS
- Publication
Journal of Shanxi Agricultural Sciences, 2023, Vol 51, Issue 10, p1162
- ISSN
1002-2481
- Publication type
Article
- DOI
10.3969/j.issn.1002-2481.2023.10.07