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- Title
人多能干细胞向多巴胺能神经元分化:异质性的安全风险.
- Authors
陈 丽; 胡 兰; 杨 柳; 申 辉; 王 埮; 赵振强; 彭雅南
- Abstract
BACKGROUND: The transplantation of human pluripotent stem cell-derived nerve cells for the treatment of Parkinson’s disease has made great progress and the relevant research is at the subclinical stage. However, there are still many problems to be solved. As one of the existing problems, heterogeneity of differentiation can cause tumor formation and dyskinesia in the host after transplantation, which brings potential and unpredictable risks to patients. OBJECTIVE: To summarize the current status of human pluripotent stem cells differentiated into dopaminergic neurons, the principle and process of differentiation design, the induction process and the research status of heterogeneous differentiation, laying a theoretical foundation for the clinical transformation of transplantation therapy. METHODS: The keywords were “iPSC AND Parkinson’s disease, induced pluripotent stem cells AND Parkinson’s disease, ES cells AND Parkinson’s disease, embryonic stem cells AND Parkinson’s disease, pluripotent stem cells AND Parkinson’s Disease” in English and Chinese, respectively. Relevant articles published from 1980 to 2018 were retrieved by the first author in the PubMed, CNKI, and WanFang. Literature addressing the treatment of Parkinson’s disease with induced pluripotent stem cell-derived neurons in recent years was reviewed, and finally 46 articles were retained. RESULTS AND CONCLUSION: A variety of induction differentiation protocols can be used to induce the differentiation of pluripotent stem cells into A9 dopaminergic neurons. Cell transplantation can promote the behavioral and limb function recovery of different Parkinson's disease models. However, in addition to the differentiation of A9 dopaminergic neurons, the current differentiation protocol products include different neurons such as A10 dopaminergic neurons and serotonergic neurons. There is no differentiation scheme that can achieve uniform differentiation. Further optimizing the differentiation conditions of human pluripotent stem cells in vitro and homogenizing differentiation into A9 dopaminergic neurons may further improve the behavioral performance of Parkinson’s disease model animals and promote the clinical transformation of the cell therapy.
- Publication
Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu, 2019, Vol 23, Issue 1, p118
- ISSN
2095-4344
- Publication type
Article
- DOI
10.3969/j.issn.2095-4344.1532