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- Title
Modelling the individual cell lag phase. Isolating single cells: protocol development.
- Authors
Francois, K.; Devlieghere, F.; Standaert, A.R.; Geeraerd, A.H.; Van Impe, J.F.; Debevere, J.
- Abstract
Abstract Aims: To develop a protocol to isolate single cells in wells of a microtitre plate, having a high certainty of individual cells, combined with a sufficient yield. Methods and Results: Single cells were obtained using 1/2 dilution series in microtitre plates. Seventy-two Lactococcus lactis dilution series were checked by plate counting. When the last five columns of the plates were observed, the chance of having one single cell was 80%, while the yield was 75 wells containing cells. A simulation model confirmed these results. This method was compared with the commonly applied method. Conclusions: This method makes it possible to combine a higher chance of having one cell in a microtitre well with a slightly higher yield. Significance and Impact of the Study: A tool is developed to isolate single cells to provide a suitable base for investigating and modelling the individual cell lag phase.
- Subjects
CELLS; MICROBIOLOGY
- Publication
Letters in Applied Microbiology, 2003, Vol 37, Issue 1, p26
- ISSN
0266-8254
- Publication type
Article
- DOI
10.1046/j.1472-765X.2003.01340.x