We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
积雪草多糖制备、结构表征及其抗氧化活性.
- Authors
乔赛凤; 宋璐杉; 刘会平; 张玉梅; 邢慧珍
- Abstract
Extract one of polysaccharides from Centella asiatica(L.)Urban by hot water extraction,and study its structural characterization and antioxidant activity. After deproteinization,dialysis and Sephadex -G200 gel column chromatography,obtaining a homogeneous component of polysaccharide of Centella asiatica(L.)Urban, named CAP. CAP with a relative molecular weight of 1.81伊106 Da,which total sugar content of(91.35依1.12)%, protein content of (0.72依0.08)%,uronic acid content of (16.88依0.53)%,and no reducing sugar. The average particle size in the solution was(325.13依6.05)nm,and the Zeta potential was(-15.24依0.38)mV,indicating that CAP was an acidic anionic polysaccharide. Ion chromatography showed that CAP was mainly composed of fucose 颐 rhamnose 颐 arabinose 颐 galactose 颐 glucose 颐 xylose 颐 mannose 颐 glucuronic acid 颐 guluronic acid,with a molar ratio of 0.01 颐 0.14 颐 0.44 颐 0.69 颐 1.00 颐 0.03 颐 0.02 颐 0.06 颐 0.11. Fourier transform infrared spectrometry and nuclear magnetic resonance indicated that CAP was a pyranoid polysaccharide with 琢 -glycosidic and 茁 -glycosidic bonds,and 5 sugar residues(A-E)were detected,with heteromeric hydrogen/carbon signals as follows:啄5.36/ 99.93,5.26/109.45,5.20/107.62,4.60/104.57,4.45/103.65. Antioxidant experiments indicated that CAP had the best effect on removing ABTS+·,while the worst effect on·OH
- Subjects
CENTELLA asiatica; POLYSACCHARIDES; ANTIOXIDANTS
- Publication
Food Research & Development, 2023, Vol 44, Issue 21, p1
- ISSN
1005-6521
- Publication type
Article
- DOI
10.12161/j.issn.1005-6521.2023.21.001