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- Title
GABA<sub>Aβ2–3</sub> immunoreactive cells in the developing chick retina
- Authors
Barros, Pedro Henrique Oliveira Cavalcanti; Calaza, Karin da Costa; Gardino, Patrıcia Franca
- Abstract
Gama-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the central nervous system (CNS). It has been shown that GABA is an important factor for CNS maturation and that its functions are mainly mediated by GABAA receptors. Thus, in order to fully comprehend the role of GABA during development, it is essential to establish the developmental features of the catalytic subunits (β) of GABAA receptor. Here, we determine the ontogenesis and neurogenesis of cells expressing β2–3 subunits of GABAA receptor (GABAAβ2–3) in the chick retina. In the ontogenetic experiments, only the immunohistochemistry for GABAAβ2–3 approach was employed. For neurogenesis a double-labeling method (autoradiography and immunohistochemistry) was applied. [H3]-thymidine was injected into eggs (2–11 days) and the embryos were sacrificed at embryonic day 19 (E19). GABAAβ2–3 immunohistochemistry was processed and then autoradiography was performed. We used a cumulative counting method to quantify the autoradiographic grains. The ontogenesis study revealed that at E9, GABAAβ2–3 immunoreactivity was restricted to the inner plexiform layer and the first cell bodies immunoreactive to GABAAβ2–3 were seen at E14. Thereafter, the number of cell bodies and the intensity of GABAAβ2–3 immunoreactivity increased until the adult pattern was established. The neurogenesis study showed that cells that will express GABAAβ2–3 were generated between E6 and E9. In addition, from E7 to E9 the rate of neurogenesis of GABAAβ2–3 immunoreactive cells quickly increases. Therefore, the detection of GABAAβ2–3 occurred only after the end of generation period of this cell population.
- Subjects
GABA; CENTRAL nervous system; DEVELOPMENTAL neurobiology
- Publication
International Journal of Developmental Neuroscience, 2003, Vol 21, Issue 1, p35
- ISSN
0736-5748
- Publication type
Article
- DOI
10.1016/S0736-5748(02)00106-5