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- Title
Expression of CrylAb Protein in a Marker-Free Transgenic Bt Rice Line and Its Efficacy in Controlling a Target Pest, Chilo suppressalis (Lepidoptera: Crambidae).
- Authors
YANAN WANG; LEI ZHANG; YUNHE LI; YANMIN LIU; LANZHI HAN; ZHEN ZHU; FENG WANG; YUFA PENG
- Abstract
A marker-free Bt transgenic rice line, mfb-MH86, was recently developed in China, which contains a cry1Ab gene driven by a ubiquitin promoter. This Bt gene confers resistance to a range of lepidopteran species, including the striped stem borer, Chilo suppressalis (Walker). The expression of Cry1Ab protein in mfb-MH86 leaves, stems and leaf sheaths (hereinafter referred to as stems), and roots was evaluated throughout the rice-growing season using an enzyme-linked immunosorbent assay. In addition, mfb-MH86 resistance to C. suppressalis, a major pest of rice, was evaluated in a laboratory bioassay with field-collected rice stems. Cry1Ab protein levels of mfb-MH86 were highest in leaves (9.71-34.09 µg/g dry weight [DW]), intermediate in stems (7.66-18.51 µg/g DW), and lowest in roots (1.95-13.40 µg/g DW). In all tissues, Cry1Ab levels in mfb-MH86 were higher in seedling and tillering stages than in subsequent growth stages. In the laboratory bioassay, mortality of C. suppressalis after 6 d of feeding on mfb-MH86 stems was 100% throughout the rice-growing season; mortality of C. suppressalis when feeding on stems of the nontransformed isoline, MH86, ranged from 15.0 to 38.3%. The results indicate that Cry1Ab protein levels in mfb-MH86 stems are sufficient to protect plants against C. suppressalis throughout the rice-growing season. Although our results are promising, further comprehensive evaluations of mfb-MH86, including field surveys, will be needed before commercial use.
- Subjects
TRANSGENIC rice; RICE disease &; pest resistance; RICE diseases &; pests treatment; CHILO suppressalis; DISEASE resistance of plants; PLANT disease research; BIOLOGICAL assay research; ENZYME-linked immunosorbent assay
- Publication
Environmental Entomology, 2014, Vol 43, Issue 2, p528
- ISSN
0046-225X
- Publication type
Article
- DOI
10.1603/EN13254