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- Title
Genetic differentiation in Pyrenophora teres f. teres populations from Syria and Tunisia as assessed by AFLP markers.
- Authors
Bouajila, A.; Zoghlami, N.; Murad, S.; Baum, M.; Ghorbel, A.; Nazari, K.
- Abstract
To investigate the level of genetic differentiation and diversity among Pyrenophora teres isolate populations originating from different agro-ecological areas of Syria and Tunisia and to determine the potential of AFLP profiling in genotyping Pyrenophora teres f . teres. In this study, AFLP markers have been employed to identify patterns of population structure in 20 Pyrenophora teres f . teres populations from Syria and Tunisia. Ninety-four isolates were studied by the use of a protocol that involved stringent PCR amplification of fragments derived from digestion of genomic DNA with restriction enzymes Eco RI and Mes I. Based on 401 amplified polymorphic DNA markers ( AFLP), variance analyses indicated that most of the variation was partitioned within rather than between populations. Genotypic diversity ( GD) was high for populations from Rihane, local landraces and different agro-ecological zones ( GD = 0·75-0·86). There was high genetic differentiation among pathogen populations from different host populations in Syria ( Gst = 0·31, ht = 0·190) and Tunisia ( Gst = 0·39, ht = 0·263), which may be partly explained by the low gene flow around the areas sampled. A phenetic tree revealed three groups with high bootstrap values (55, 68, 76) and reflected the grouping of isolates based on host, or agro-ecological areas. AFLP profiling is an effective method for typing the genetically diverse pathogen Pyrenophora teres f. teres. Significance and Impact of the Study The study represents a comparative analysis of the genetic diversity in P. teres isolates from two countries spanning two continents and also shows that several distinct P. teres genotypes may be found in a given environment. The implications of these findings for Pyrenophora teres f. teres evolutionary potential and net blotch-resistance breeding in Syria and Tunisia were also discussed.
- Subjects
TUNISIA; SYRIA; PYRENOPHORA teres; AMPLIFIED fragment length polymorphism; DNA restriction enzymes; GENETIC markers; BIOLOGICAL variation; GENE flow
- Publication
Letters in Applied Microbiology, 2013, Vol 56, Issue 6, p389
- ISSN
0266-8254
- Publication type
Article
- DOI
10.1111/lam.12029