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- Title
A simple protocol to purify fresh nuclei from milligram amounts of meristematic pea root tissue for biochemical and flow cytometry applications.
- Authors
Chiatante, Donato; Brusa, Paola; Levi, Marisa; Sgorbati, Sergio; Sparvoli, Elio
- Abstract
A simple protocol to purify fresh nuclei from very small amounts (mg) of Pisum sativum (cv. Lincoln) root tissue is presented. The protocol is reliable and has been repeated many times; it can be performed within a few hours and needs only a simple modification of common glassware for tissue homogenization. Similar yields of purified nuclei are obtained both with meristematic and adult root tissues using a detergent-free glycerol buffer. Purified nuclei can be stored at -20°C for several weeks without any appreciable loss of integrity. The high yield of purified nuclei enables us to use our preparations for biochemical and cytometric investigations.
- Subjects
CELL nuclei; FLOW cytometry; PEAS; MERISTEMS; PLANT roots; PLANT cells &; tissues
- Publication
Physiologia Plantarum, 1990, Vol 78, Issue 4, p501
- ISSN
0031-9317
- Publication type
Article
- DOI
10.1111/j.1399-3054.1990.tb05233.x