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- Title
Phosphotungstic Acid Capped Gold Nanoparticles for Spectrophotometric Determination of Uric Acid in Diagnostics of Gout.
- Authors
Raj, Vidya; Alex, Saji
- Abstract
A simple colorimetric method for the estimation of serum uric acid (UA), a marker of gout (a form of inflammatory arthritis), is proposed. The present study focuses on the use of phosphotungstic acid (PTA) modified gold nanoparticles (PAuNPs) as a sensing platform for UA detection making it an attractive alternative to the existing technique involving elaborate instrumentation. PAuNPs were prepared by irradiation of chloroauric acid and PTA by UV light. The binding of PAuNPs with UA was characterized by a visible color change of the solution followed by the red shift in the plasmon absorption band. The basic principle applied here is the formation of allantoin and carbon dioxide by the reaction between PTA and UA. The method shows linearity between 1.7–14 mg/dL with the detection limit of 1.2 mg/dL. The color change and the variation in the absorbance of PAuNPs were highly specific for UA in the presence of other major interferents. The method was successfully applied for the detection of UA in real samples of urine and blood. The method is very simple and could be adopted by any person, which enables patients to have a self-checking method at home before seeking medical attention. This type of sensing kit would be highly beneficial in rural health care centers, where high-tech diagnostic tests are unavailable for most people.
- Subjects
PHOSPHOTUNGSTIC acids; URIC acid; GOLD nanoparticles; GOUT; RURAL health services; REDSHIFT
- Publication
Journal of Analytical Chemistry, 2022, Vol 77, Issue 10, p1267
- ISSN
1061-9348
- Publication type
Article
- DOI
10.1134/S1061934822100124