We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Kinetic Analysis Misinterpretations Due to the Occurrence of Enzyme Inhibition by Reaction Product: Comparison between Initial Velocities and Reaction Time Course Methodologies.
- Authors
Fernandes, Joana M. C.; Dias, Albino A.; Bezerra, Rui M. F.
- Abstract
Featured Application: The integrated Michaelis–Menten equation (IMME) provides a viable and accurate methodology for enzyme kinetic studies when the product is also an inhibitor. This methodology is important in a wide range of applications, including in the preclinical kinetic assays of potential drug candidates. This methodology is a powerful tool for overcoming the inaccuracies associated with archaic curve linearization methods for initial velocities determination mainly when the reaction product is an enzyme inhibitor. The Michaelis–Menten equation (MME) has been extensively used in biochemical reactions, but it is not appropriate when the reaction product inhibits the enzyme. Under these circumstances, each determined initial velocity, v0, is one experimental point that actually belongs to a different MME because enzymatic product inhibition occurs as the reaction starts. Furthermore, the inhibition effect is not constant, since the concentration of the product inhibitor rises as time increases. To unveil the hidden enzyme inhibition and to simultaneously demonstrate the superiority of an integrated Michaelis–Menten equation (IMME), the same range of data points, assuming product inhibition and the presence of a second different inhibitor, was used for kinetic analysis with both methodologies. This study highlights the superiority of the IMME methodology for when the enzyme is inhibited by the reaction product, giving a more coherent inhibition model and more accurate kinetic constants than the classical MME methodology.
- Subjects
MICHAELIS-Menten equation; ENZYMES; ENZYME inhibitors; VELOCITY; REACTION time
- Publication
Applied Sciences (2076-3417), 2022, Vol 12, Issue 1, p102
- ISSN
2076-3417
- Publication type
Article
- DOI
10.3390/app12010102