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- Title
Patient‐derived organoids in cellulosic sponge model chemotherapy response of metastatic colorectal cancer.
- Authors
Xu, Yanjie; Chen, Jianjun; Huang, Yizhou; Luo, Yang; Hsieh, An‐Chih; Chen, Jianyi; Li, Han; Wei, Xunbin; Gao, Wei‐Qiang; Zhong, Ming; Zhang, Yan
- Abstract
Patient-derived organoids (PDOs) closely recapitulate human colorectal cancer biology and have recently emerged as preclinical models for personalized therapy design.1 However, PDOs cultured in Matrigel (PDOs SP Matrigel sp ) failed to predict outcome for treatment with the first-line FO chemotherapeutic regimen (5-fluorouracil plus oxaliplatin) for metastatic colorectal cancer (mCRC) patients.2 In this study, we establish in vitro culture conditions of mCRC-PDOs in a hydroxypropyl cellulose allyl conjugated with collagen (HA-Coll sponge),3 and utilized this system to examine the drug sensitivity of FO regimen (Figure 1A). PDOs in HA-Coll sponge (PDOs SP Sponge sp ) proliferated at the same level as PDOs SP Matrigel sp in the next generation (Figure 2B and C, Figure S1C and D). Conclusively, our results indicated that PDOs SP Sponge sp remained the original epithelial state of parental tumor tissues, which is important for the drug-sensitivity of FO, but not for irinotecan. To further confirm these results, we knocked out I LMNA i in PDOs SP Sponge sp and overexpressed I LMNA i in PDOs SP Matrigel sp to perform the drug response assay.
- Subjects
METASTASIS; COLORECTAL cancer; ORGANOIDS; CANCER chemotherapy; EPITHELIAL-mesenchymal transition
- Publication
Clinical & Translational Medicine, 2021, Vol 11, Issue 1, p1
- ISSN
2001-1326
- Publication type
Article
- DOI
10.1002/ctm2.285