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- Title
Taurine reduces FK506-induced generation of ROS and activation of JNK and Bax in Madin Darby canine kidney cells.
- Authors
Jeon, Seol-Hee; Park, Hye-Min; Kim, Shang-Jin; Lee, Mun-Young; Kim, Gi-Beum; Rahman, Md. Mizanur; Woo, Jeong-Nam; Kim, In-Shik; Kim, Jin-Shang; Kang, Hyung-Sub
- Abstract
The immunosuppressive compound FK506 has been successfully used in kidney and liver transplant recipients. However, the compound can induce significant side effects on kidney function. Taurine is a potent free radical scavenger that attenuates a variety of renal diseases that are the consequence of excessive oxygen free radical damage. The purpose of this study was to investigate FK506-mediated death of Madin Darby canine kidney (MDCK) cells, in relation to reactive oxygen species (ROS) production. We determined the calcium (Ca2+) and magnesium (Mg2+) concentration in cultured MDCK cells by microfluorescence techniques and the level of activation of c-Jun-N-terminal kinase (JNK), extracellular signal regulated kinases (ERK), Bcl-2 and Bax proteins by Western blot. Treatment with 10 µM FK506 induced apoptosis in MDCK cells by increasing the level of intracellular ROS and Ca2+ and by decreaseing the level of intracellular Mg2+. This increase in intracellular ROS promoted JNK and Bax activation, which increased FK506-induced MDCK cell death. Taurine reduced the FK506-induced generation of ROS and activation of JNK and Bax. The results indicate that taurine can prevent FK506-induced kidney toxicity.
- Subjects
NEPHROTOXICOLOGY; FOCAL adhesion kinase; REACTIVE oxygen species; KIDNEY diseases; KIDNEY function tests; TACROLIMUS; MICROBIAL viability counts
- Publication
Human & Experimental Toxicology, 2010, Vol 29, Issue 8, p627
- ISSN
0960-3271
- Publication type
Article
- DOI
10.1177/0960327109359019