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- Title
Menin orchestrates hepatic glucose and fatty acid uptake via deploying the cellular translocation of SIRT1 and PPARγ.
- Authors
Liu, Tingjun; Li, Ranran; Sun, Lili; Xu, Zhongjin; Wang, Shengxuan; Zhou, Jingxuan; Wu, Xuanning; Shi, Kerong
- Abstract
Background: Menin is a scaffold protein encoded by the Men1 gene, which interacts with various transcriptional proteins to activate or repress cellular processes and is a key mediator in multiple organs. Both liver-specific and hepatocyte-specific Menin deficiency promotes high-fat diet-induced liver steatosis in mice, as well as insulin resistance and type 2 diabetic phenotype. The potential link between Menin and hepatic metabolism homeostasis may provide new insights into the mechanism of fatty liver disease. Results: Disturbance of hepatic Menin expression impacts metabolic pathways associated with non-alcoholic fatty liver disease (NAFLD), including the FoxO signaling pathway, which is similar to that observed in both oleic acid-induced fatty hepatocytes model and biopsied fatty liver tissues, but with elevated hepatic Menin expression and inhibited FABP1. Higher levels of Menin facilitate glucose uptake while restraining fatty acid uptake. Menin targets the expression of FABP3/4/5 and also CD36 or GK, PCK by binding to their promoter regions, while recruiting and deploying the cellular localization of PPARγ and SIRT1 in the nucleus and cytoplasm. Accordingly, Menin binds to PPARγ and/or FoxO1 in hepatocytes, and orchestrates hepatic glucose and fatty acid uptake by recruiting SIRT1. Conclusion: Menin plays an orchestration role as a transcriptional activator and/or repressor to target downstream gene expression levels involved in hepatic energy uptake by interacting with the cellular energy sensor SIRT1, PPARγ, and/or FoxO1 and deploying their translocations between the cytoplasm and nucleus, thereby maintaining metabolic homeostasis. These findings provide more evidence suggesting Menin could be targeted for the treatment of hepatic steatosis, NAFLD or metabolic dysfunction-associated fatty liver disease (MAFLD), and even other hepatic diseases. Highlights: Higher Menin facilitates hepatic glucose uptake, but restrains hepatic fatty acid uptake. Conversely, lower Menin has the opposite effect. Menin interacts with PPARγ and/or FoxO1, as well as SIRT1, thereby targeting downstream gene expression. Through its recruitment of various proteins, Menin facilitates their translocations within the nucleus and cytoplasm. Menin serves as a key regulator of hepatic metabolism homeostasis. Menin expression is elevated in both induced fatty hepatocyte model and fatty liver tissues.
- Subjects
SIRTUINS; NON-alcoholic fatty liver disease; FATTY liver; FATTY acids; INSULIN; GENE expression
- Publication
Cell & Bioscience, 2023, Vol 13, Issue 1, p1
- ISSN
2045-3701
- Publication type
Article
- DOI
10.1186/s13578-023-01119-y