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- Title
Immunocytochemical detection of ornithine decarboxylase (ODC) in paraffin-embedded tissues as a possible prognostic indicator for oral lesions.
- Authors
Williams, H K; Springall, D R; Bhatnagar, M; Polak, J M
- Abstract
Intracellular levels of ornithine decarboxylase (ODC) are raised following mitogenic stimulus and in neoplasia. Because lesions of the oral cavity are often difficult to assess histologically, we have determined the value of immunocytochemical detection of ODC as a prognostic indicator in 74 routinely fixed and paraffin-embedded oral biopsies using peroxidase-antiperoxidase and immunogold-silver staining. The latter proved more sensitive, yielding positive reactions in 32 of 43 oral carcinomas (11/14 well differentiated, 16/21 moderately differentiated and 3/5 poorly differentiated) and 7/11 potentially malignant lesions, compared with 19/45 carcinomas and 1/15 potentially malignant lesions, by peroxidase anti-peroxidase. Hyperplastic lesions (n = 7) and normal non-keratinized buccal mucosa (n = 7) were all negative. Follow-up was possible in 13 of the carcinoma patients. Of 7 positive ODC reactions but clinically node-free at biopsy, 2 died and 2 had recurrences within 3 years, whereas all of 6 with no immunoreactivity were symptom-free after 3-5 years. Immunostaining for ODC may be helpful for the prognostic assessment of routinely processed oral lesions and in choosing treatment.
- Subjects
ENZYME analysis; HYPERPLASIA; ANIMAL experimentation; BENZOPYRANS; CHI-squared test; COMPARATIVE studies; DYES &; dyeing; HISTOLOGICAL techniques; IMMUNOENZYME technique; IMMUNOHISTOCHEMISTRY; KIDNEYS; ORAL leukoplakia; RESEARCH methodology; MEDICAL cooperation; MICE; MOUTH tumors; ORAL mucosa; PROGNOSIS; RATS; RESEARCH; SQUAMOUS cell carcinoma; TESTOSTERONE; THRUSH (Mouth disease); EVALUATION research; CARCINOMA in situ; FLUORESCENT dyes
- Publication
Journal of Oral Pathology & Medicine, 1995, Vol 24, Issue 7, p322
- ISSN
0904-2512
- Publication type
journal article
- DOI
10.1111/j.1600-0714.1995.tb01193.x